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. 2019 Mar 21;13(3):e0007256. doi: 10.1371/journal.pntd.0007256

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© 2019 Fassolari, Alonso

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) RT-PCR of the three TcAUKs and Actin (housekeeping control) in epimastigotes (E), trypomastigotes (T) and amastigotes (A) of T. cruzi. (-): PCR negative control. I and II indicate the specific amplification products of similar length obtained for TcAUK3 (B) Nucleotide sequence of the 5´ Untranslated Region of each TcAUK mRNA. The Spliced Leader sequence and the starting ATG codon followed by the initial sequence of the genes (CDS) are indicated. I and II reference to the bands indicated in (A). Underlined in TcAUK3 is the region of the 5´ UTR absent in one of the two amplification products. (C) Immunoblot of TcAUK1 in total cell extracts from Epimastigotes (E), Trypomastigotes (T) and Amastigotes (A).