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. 2019 Mar 21;13(3):e0007256. doi: 10.1371/journal.pntd.0007256

Fig 4. TcAUK1 localization in the different forms of T. cruzi.

Fig 4

(A) Epimastigote forms at different points of the cell cycle (1F1K1N, 2F2K1N, 2F2K2N) were co-immunostained with rabbit antiserum to TcAUK1 and mouse KMX-1 for TcAUK1 and mitotic spindle, respectively. Yellow arrowhead indicates cells where the nuclei have not segregated yet, and white arrowhead points cells where both nuclei are segregating and (B) Amastigote and Trypomastigote forms isolated from culture supernatants were immunostained with rabbit antiserum to TcAUK1. (C) Infected Vero cells were stained with rhodamine-conjugated phalloidin for actin filaments and intracellular parasites were immunostained with rabbit antiserum to TcAUK1. In all cases, to dye DNA structures–nucleus (n or N) and kinetoplast (k)–cells were counterstained with DAPI. White squares point the magnified regions.