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. 2019 Apr 1;9:5446. doi: 10.1038/s41598-019-41940-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential expression analysis of the Tmem67^−/− post-natal cerebellum identifies a cluster of co-regulated homeobox transcription factors. (a) Heat map representing relative gene expression levels in early postnatal (P0) cerebella from matched littermate Tmem67^+/+ and Tmem67^−/− animals (n = 3 biological replicates) for the indicated genes. All statisitically significant differentially expressed genes (total n = 41; Benjamini-Hochberg p-value adjusted for false discovery rate p_adj < 0.01) in all three data-sets are represented. Up-regulated genes in Tmem67^−/− mutants are coloured in dark-red/brown, with the co-regulated cluster of Hox genes indicated by orange arrowheads. Down-regulated genes are coloured in orange/yellow, with Tmem67 indicated by the brown arrowhead. The DESeq2 output table for significant differentially expressed genes is shown in Supplementary Table S1. (b) Volcano plot displaying the 41 genes differentially-expressed in early postnatal Tmem67^+/+ and Tmem67^−/− cerebella that pass statistical cut-offs of false discovery rate (FDR) p_adj < 0.05 and absolute log₂fold change (FC) > = 1. The vertical axis (y-axis) corresponds to the statsticial significance of mean expression log₁₀(p_adj value) and the horizontal axis (x-axis) displays the log₂ fold-change values. Positive log₂fold-changes (red points) indicate up-regulated expression in the Tmem67^−/− mutant compared to the Tmem67^+/+ wild-type data-sets. Negative log₂fold-changes (cyan points) indicate down-regulated expression in Tmem67^−/−. Genes discussed in the main text are highlighted with coloured text and larger data points.