Table 2. Human AChR gating and binding free energy changes.

Site	Agonist	ΔG1	ΔGR	ΔGR*
α–ε	ACh	2.8	−5.6	−11.2
	CCh	3.3	−5.1	−10.4
	TMA	3.7	−4.4	−9.1
	Cho	5.1	−3.4	−6.7
α–δ	ACh	3.2	−5.3	−10.5
	CCh	3.8	−4.6	−9.2
	TMA	4.2	−4.2	−8.5
	Cho	5.5	−3.2	−6.1
α–δ	Epi	3.7	−7.0	−11.7
	Ebx	4.0	−5.9	−10.4
	Anx	4.8	−5.0	−8.7
	Aza	5.4	−4.1	−7.1
α–γ	ACh	1.7	−5.2	−13.1
	CCh	2.9	−5.4	−12.1
	TMA	3.7	−4.7	−10.6
	Cho	4.6	−4.1	−9.0

All values are kilocalories per mole. ΔG₁, gating with one bound agonist; ΔG_R, binding to the resting conformation; ΔG_R*, binding to the active conformation (Fig. 1 b).