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. 2019 Jan 22;151(4):518–531. doi: 10.1085/jgp.201812270

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© 2019 Chen et al.

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Figure 3. — Side-chain charge modifications of the residue at position 584 of TMEM16A. (A and B) I-V curves of WT_16A (A) and K584H_16A (B) with 400 µM [Ca²⁺]_i at pH 5.4 (blue), 6.4 (pink), and 7.4 (green). Insets show averaged rectification indices (n = 6, *, P < 0.05 and **, P < 0.005 by Student’s t test). (C and D) Manipulation of the side-chain charge of K584C_16A by MTSES (C) and MTSET (D) modifications. In each panel, recording traces of MTS modifications of WT_16A and K584C_16A at −40 mV are depicted on the left and middle, respectively. The dashed line represents zero current level. The structures of the MTS molecules and the I-V curves of K584C_16A before and after MTS modification are shown on the right. The RI values are displayed next to each I-V curve. The RI values after MTSES (n = 9) and MTSET (n = 6) modifications were significantly different from those before the modifications (P < 0.05, paired Student’s t test).