Table 1. Ca²⁺-dependent activation of WT TMEM16F and aromatic Q559 mutants using the three-pulse protocol.

Channel	Ca2+ dose–response
	K1/2 (µM)	Hill coefficient
WT16Fa	34 ± 3	1.2 ± 0.1
Q559F16F	10 ± 1	1.5 ± 0.1
Q559Y16F	7.9 ± 0.9	1.3 ± 0.1
Q559H16F	3.2 ± 0.6	1.9 ± 0.2
Q559W16F	2.2 ± 0.7	0.9 ± 0.2

aChannel likely in partially rundown state.