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. 2019 Jan 29;151(4):505–517. doi: 10.1085/jgp.201812195

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© 2019 Bartoccioni et al.

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Figure 1. — BasC purification and reconstitution in PLs. (A) SEC of DDM-solubilized Ni²⁺-NTA purified BasC-GFP protein. mAUs, milli absorbance units. (B) Coomassie blue–stained gel and in-gel fluorescence of purified BasC. Purification of His-tagged BasC-GFP by nickel affinity chromatography with or without a further SEC step. Coomassie blue–stained (left) and in-gel fluorescence (right) SDS-PAGE (12% polyacrylamide gel) of the different purification steps is shown: Ni²⁺-NTA purified BasC-GFP (lane 1), BasC reconstituted in PLs (lane 2), and SEC-purified BasC (lane 3). BasC-GFP migrates as a prominent band at ∼50 kD.