Figure 1.

Molecular evidence for Ano1 and CaCC current in LMCs. Representative RT-PCR, resolved by agarose gel electrophoresis, for Ano1 (TMEM16a) (A) and TMEM16b (B) mRNA expression in lymphatic IALVs, LMCs, with lung and brain tissue as positive controls. (C) Western blot of collected IALVs and lung lysate with both the monomeric and dimer forms of Ano1 observed. Representative immunofluorescence maximal projection of an IALV stained anti-Ano1 (D) and with anti-SMA (E) to delineate Ano1 expression in LMCs (F, merge) at 20× objective magnification with the corresponding negative control (G). Representative immunofluorescence maximal projection of a popliteal lymphatic vessel isolated from an Ano1-GFP reporter mouse was stained with anti-GFP conjugated to AF488 (H) and anti-SMA conjugated to Cy3 (I) to further demonstrate LMC expression of Ano1 (J, merge).