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. 2019 Mar 12;151(4):532–554. doi: 10.1085/jgp.201812294

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© 2019 Zawieja et al.

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Figure 11. — Ca²⁺ flash analysis in pressurized IALV from SMMHC-CreER^T2-GCaMP6f-Ano1KO mice. Pressurized (3 cm H₂O) control SMMHC-CreER^T2-GCaMP6f (A) and SMMHC-CreERT2-GCaMP6f-Ano1KO (B) vessels were imaged at 20–33 fps with a 20× objective. Image sequences were made into maximum z-stack projections to create a single-cell mask to assess Ca²⁺ intensity over baseline (F/F₀). Ca²⁺ flashes (#) are indicative of action potential–generated Ca²⁺ influx through LTCCs, although spontaneous and local Ca²⁺ signals are also evident (^). Summary data for Ca²⁺ flash frequency (C), peak Ca²⁺ intensity (D), time to reach peak Ca²⁺ (E), Ca²⁺ flash duration (F), and the area under the curve (G) were measured. *, P < 0.05 comparing SMMHC-CreER^T2-Ano1KO versus Ano1fl/fl with n = 9 for control, n = 8 for SMMHC-CreER^T2-Ano1KO.