Table 1. Primers used for qPCR and RT-PCR.

qPCR primers
Gene	Accession no.		Catalog Number	Description
Ano1	NM_178642		IDT Mm.PT.58.12522115	Mouse Ano1 TaqMan probe IDT
SMA	NM_007392		IDT Mm.PT.58.16320644	Mouse α-actin TaqMan probe IDT
β-Actin	NM_007393		IDT Mm.PT.58.33257376.gs	Mouse Actb TaqMan probe IDT
End point RT-PCR
Gene	Accession no.	Strand	Sequence / Catalog Number	Amplicon (bp)
Ano1	NM_178642	F	5′-GGC​ATT​TGT​CAT​TGT​CTT​CCA​G-3′	141
		R	5′-TCC​TCA​CGC​ATA​AAC​AGC​TC-3′
TMEM16B	NM_153589	F	5′-TTT​ATG​ATT​GCC​CTG​ACG​TTC​TC-3′	141
		R	5′-GAG​GTT​GAT​GAT​GAC​TGC​TGT​T-3′
Bestrophin 1	NM_011913	F	5′-ACA​CAA​CAC​ATT​CTG​GGT​GC-3′	123
		R	5′-CGC​AAA​GTA​CAC​ACC​TCA​TTC​A-3′
Bestrophin 2	NM_001130194.1	F	5′-TCC​AGG​GCT​CCA​CCT​TTG​ATA​TAG-3′	101
		R	5′-AAA​GTC​CCC​GTG​AAC​CTC​TCC-3′
CLCA1	NM_017474	F	5′-GAA​ATT​CCC​CAG​CCC​TGT​AAC​AG-3′	144
		R	5′-TCG​GCA​CCT​GCT​CCG​TTA​TC-3′
CLCA3A1	NM_009899	F	5′-GTG​GAC​CAG​CCT​TTC​TAC​ATG​TCT​AG-3′	114
		R	5′-TGT​GAC​ACA​GTT​GCC​TCT​CTC​A-3′
NKCC1 (Slc12a2)	NM_009194	F	IDT Mm.PT.58.32659494	131
		R
NKCC2 (Slc12a1)	NM_001079690	F	IDT Mm.PT.58.29984258	118
		R

List of all the primer sequences, their corresponding gene accession no. used for their design, and the predicted amplicon size. F, forward; R, reverse.