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. 2019 Mar 20;43(5):2164–2176. doi: 10.3892/ijmm.2019.4143

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Copyright: © Tanaka et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Plagl1 expression in embryonic murine brains and localization of PLAGL1 in hypocretin neurons of the embryonic murine hypothalamus. The Plagl1 RNA probe detected Plagl1 expression in periventricular zones and the hypothalamus at E10-E13 by in situ hybridization (red arrowheads) (magnification, x20). Murine HCRT is visualized by staining with Alexa Fluor^®488 (green) and murine PLAGL1 is visualized by staining with Alexa Fluor^® 594 (red) at E14 and E18. 3vt, third ventricle; E, embryonic day; fv, forebrain vesicle; HCRT, hypocretin; mv, mesencephalic vesicle; PLAGL1, pleomorphic adenoma gene-like 1; tv, telencephalic vesicle.