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. 2019 Mar 4;43(5):2086–2102. doi: 10.3892/ijmm.2019.4120

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Copyright: © Yin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Validation of the TNF-α/IEC-6 model, IEC-6 cell viability and BMMSC chemotaxis. (A) Protein and mRNA content of ZO-1 in IEC-6 cells and TNF-α/IEC-6. (B) IEC-6 viability in each of the groups was detected. (C) BMMSC migration; the number of migrated BMMSCs in the Ad-CXCR3/MSCs + L and Ad-(CXCR3 + HO)/MSCs + L groups were all higher than in the other groups. (D) Migrated BMMSCs across Transwell membranes (indicated by black arrows): (a) L group, (b) MSC + L group, (c) Ad/MSCs + L group, (d) Ad-HO/MSCs + L group, (e) Ad-CXCR3/MSCs + L group, and (f) Ad-(CXCR3 + HO)/MSCs + L (scale bar, 100 µm). (E) A large number of CXCR3-modified BMMSCs migrated together at 1 h, whereas a (F) small number of BMMSCs without the CXCR3 gene modified began to migrate at 4 h (indicated by white arrows; scale bar, 50 µm). ^*P<0.05. IEC-6, intestinal epithelial crypt cell line-6; ZO-1, zonula occludens-1; TNF-α; tumor necrosis factor-α; BMMSCs, bone marrow mesenchymal stem cells; Ad, adenovirus; CXCR3, CXC-chemokine receptor CXCR3; HO-1, heme oxygenase-1; L, lymphocytes; S, S203580.

Validation of the TNF-α/IEC-6 model, IEC-6 cell viability and BMMSC chemotaxis. (A) Protein and mRNA content of ZO-1 in IEC-6 cells and TNF-α/IEC-6. (B) IEC-6 viability in each of the groups was detected. (C) BMMSC migration; the number of migrated BMMSCs in the Ad-CXCR3/MSCs + L and Ad-(CXCR3 + HO)/MSCs + L groups were all higher than in the other groups. (D) Migrated BMMSCs across Transwell membranes (indicated by black arrows): (a) L group, (b) MSC + L group, (c) Ad/MSCs + L group, (d) Ad-HO/MSCs + L group, (e) Ad-CXCR3/MSCs + L group, and (f) Ad-(CXCR3 + HO)/MSCs + L (scale bar, 100 µm). (E) A large number of CXCR3-modified BMMSCs migrated together at 1 h, whereas a (F) small number of BMMSCs without the CXCR3 gene modified began to migrate at 4 h (indicated by white arrows; scale bar, 50 µm). ^*P<0.05. IEC-6, intestinal epithelial crypt cell line-6; ZO-1, zonula occludens-1; TNF-α; tumor necrosis factor-α; BMMSCs, bone marrow mesenchymal stem cells; Ad, adenovirus; CXCR3, CXC-chemokine receptor CXCR3; HO-1, heme oxygenase-1; L, lymphocytes; S, S203580.