Siglec-8 endocytosis occurs via a clathrin- and dynamin-dependent pathway. Siglec-8 surface expression on eosinophils was measured as in Fig 1 after 120 minutes of incubation at 37°C after preincubation with the indicated concentrations of sucrose (A), amantadine (B), dynasore (C), nystatin (D), or LY294002 (E) and is expressed as a percentage of surface expression at 0 minute. Data represent the means ± SDs of 3 (A, B, C, and D) or 5 (E) independent experiments. *P < .05, **P < .01, and ***P < .001.