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. 2019 Mar 4;5(4):e437. doi: 10.1097/TXD.0000000000000876

FIGURE 9.

FIGURE 9

Hepatic necrosis and EC integrity after transplantation. CD31 integrity score showed higher score in the Steen vs Gelofusine vs WB groups compared with SCS group (3.33 ± 0.51 vs 3.16 ± 0.75 vs 3.16 ± 1.16 vs 2 ± 0.63; P < 0.01) (A). Hyaluronic acid levels were significantly lower in the Steen, Gelofusine, and SCS groups when compared with the WB group (1257 ± 358 ng/μL vs 1501 ± 143 ng/μL vs 1280 ± 151 ng/μL vs 2925 ± 912 ng/μL; P = 0.01) (B). Of note, hematoxylin and eosin analysis demonstrated a significantly lower percentage of necrosis at POD3 in Steen group compared with Gelofusine (12.5 ± 6% vs 20 ± 4.5%; P = 0.03) and SCS group (12.5 ± 6% vs 25 ± 5%; P < 0.01) (C). All the animals in the perfusion groups showed preserved bile duct structure and epithelium (Steen vs Gelofusine vs WB). In contrast, 2 of 6 animals showed alternated areas of epithelium loss and distorted structure in the bile duct (D). Six experiments were performed per group (n = 6). Student t test was used for all the analyses. EC, endothelial cell; SCS, static cold storage; WB, whole blood.