Fig. 5.

Metastatic Tumor-in-a-Dish (TiD) organoids offer a novel drug testing platform. (A) Dose-response curves for colon cancer cells HCT116, SW480 and DLD1 in mTiD cultures after 72 h of exposure to 5FU, irinotecan, oxaliplatin or SN38 are depicted. HCT116 cells were less sensitive to 5FU and DLD1 were less sensitive to oxaliplatin compared to the other agents. IC50 values were determined in dissociated mTiD cultures after 72 h of drug treatment by flow cytometry for GFP-positive colon cancer cells. (B) Colon cancer cell lines grown in 2D are sensitive to chemotherapy agents. HCT116, SW480 and DLD1 were grown in 2D culture and treated with 5FU, Oxaliplatin, Irinotecan or SN38 for 72 h. Cytotoxicity was assessed using hexosaminidase assay. Dose and time dependent cytotoxicity was observed in all three cell lines. IC50 values of colon cancer cells in 2D cultures in response to chemotherapeutic agents over 72 h are depicted. IC50 values were determined in 2D cultures using the hexosaminidase assay. (C) HCT116, (D) SW480 or (E) DLD1 were grown as single cell type 3D cultures and treated with chemotherapeutic agents. Brightfield images were captured after 72 h. (F) mTiD organoids containing mCherry-labeled ovarian cancer cell lines A2780 or A2780/C30-mCherry were imaged by bright field and immunofluorescence. Flow sorted mTiD cultures demonstrate 21.3 to 16.2% tumor (right panels). (G) mTiD-ovarian cancer cells were treated with 5FU and platinum drug (cisplatin, oxaliplatin and carboplatin) for 72 h. A2780\C30 cells are resistant to all the agents compared to A2780. (H) A2780 and A2780/C30 were grown in 2D cultures and treated with 5FU, cisplatin, oxaliplatin and carboplatin for 72 h. Cytotoxicity was assessed using hexosaminidase assay. Dose and time dependent cytotoxicity was observed in both cell lines.