Figure 2. Protein expression and activity of c-MYC in prostates of Foxp3/Tsc1 double-cKO mice.
A and B. Protein expressions and phosphorylation status of total and pT58/pS62-c-MYC and activation of mTOR as determined by IHC and Western blots of prostate tissues of Foxp3 cKO, Tsc1 cKO, or Foxp3/Tsc1 double cKO mice at 12 weeks of age. C. Relative mRNA levels of c-MYC target genes as a percentage of Hprt expression in micro-dissected prostate epithelial cells as determined by qPCR in the mice at 12 weeks of age. * p < 0.05 by one-way ANOVA followed by protected least-significant difference test between groups. T58: threonine 58; S62: serine 62; p: phosphorylation; cKO, prostate conditional knockout. All experiments were repeated two times.