Figure 6.
64B reduced UM tumor growth, circulating tumor cells and metastasis and inhibited c-Met and CXCR4 expression in hypoxic/HIF-1α expressing tumors in eye and liver in a uveal melanoma mouse model. 92.1-GFP UM cells (2× 106/ eye) were injected in six-week-old female nu/nu mice [JAX Lab; Strains: Outbred athymic nude; J:NU (007850)] (10 mice/group). On day 1, mice received either vehicle control or 120mg/kg/i.p of 64B 5 days per week. Eyes were enucleated on day 9. A, Representative H&E pictures (left) and immunofluorescence analysis for CXCR4 and c-Met (right) in tumor-bearing eyes. B, Quantification of tumor size on 6 representative mice/group). C, Quantification of CXCR4 and cMet positive cells from (A). D, HIF-1α and Pimo immunofluorescence staining in representative control and 64B-treated tumor-bearing eyes (5 mice/group). E, Representative flow cytometry analysis (left) and quantification (right) of circulating tumor cells (GFP-labeled) in mice blood collected on day 9 (6 mice/group). F, Representative pictures of HIF-1α and Pimo immunofluorescence staining in livers from vehicle and 64B-treated mice (7 mice/group). * p < 0.05; ** p < 0.01; ***, p < 0.001.