TABLE 1:

shRNA library and screening criteria

Human Module	Gene Targets	# of target mRNAs	shRNA complexity
1	Signaling pathway targets	5,043	27,500
2	Disease-associated targets	5,412	27,500
3	Cell surface, extracellular, DNA-binding targets	4,922	27,500

Hit Criteria	Definition		Module #	# of hits
Fold increase	≥1.55		1	65
# clones	≥3		2	139
Replicates	≥2 of 3		3	198
p38 regulated	Up- or down-regulated
Metastasis	Involved?