. Author manuscript; available in PMC: 2019 Oct 1.

Published in final edited form as: Cerebellum. 2019 Apr;18(2):245–254. doi: 10.1007/s12311-018-0987-5

Table 1.

List of primers used for cloning of different domains of TRIM67

F1	F2	F3
FW: GGCGGTGT AGCCTG GTGGCT CCCGC	FW1: ATTCAATG GCCATCTGCCAG CTGTGC	FW: ATTCAATG AAAGAG AGGGAG CACAAGC
RV: CAGGCTAC ACCGCC GGGGCT GCAGC	RV1: AGATGGCC ATTGAATTCAAG TCCTCTTCAGAA	RV: TCTCTTTC ATTGAATTCAAG TCCTCTTCAGA
	FW2: AGGTGACC TAGCCTGGTGGC TCCCGC
	RV2: CAGGCTAG GTCACCTTGGTG AGCAGC

FW, forward primer; RV, reverse primer. For F2 cloning, we performed two successive PCRs using respectively FW1, RV1 and FW2, RV2 primers for each step