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FR900359 inhibits GTPγS binding to Gαq. Dose-dependent inhibition of GTPγS binding to Gαq and Gαq-Q209L. FR-preincubated Gαq and Gαq-Q209L were incubated with GTPγS for 120 min in the presence of 300 mM (NH4)2SO4 and analyzed for GTPγS binding. Error bars represent the mean ± SEM from 3 experiments.
