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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Mol Cancer Res. 2019 Jan 17;17(4):929–936. doi: 10.1158/1541-7786.MCR-18-0367

Figure 3.

Figure 3

Ezrin regulates actin dynamics in PDAC CSCs. A, F-actin (phalloidin) and G-actin (DNAse1) staining of L3.6pl cells expressing either Ezrin shRNA or control luciferase shRNA after 72 hrs of doxycycline-induced knock-down (bar = 10 μm). B, Flow cytometry for F-actin (phalloidin) and G-actin (DNAse1) in L3.6pl cells after 3 days of Ezrin shRNA induction. Error bars represent SD from 10 independent experiments. C, Colony formation of L3.6pl cells with shRNA against Ezrin treated with Y-27632 and/or doxycycline. Y-27632 treatment was initiated 24hrs after doxycycline induction. Error bars represent SD from 5 independent experiments. *, 0.01<p<0.05; **, 0.001<p<0.01; ***, 0.0001<p<0.001. NS, not significant