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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Cancer Res. 2019 Jan 31;79(7):1331–1342. doi: 10.1158/0008-5472.CAN-18-1653

Figure 4. Acetyltransferase activity of p300 is essential to LLC-induced muscle wasting.

Figure 4.

(A) Cancer-induced activation of catabolic pathways in muscle requires p300 with intact acetyltransferase activity. A dominant negative p300 mutant (DN-p300) with defective acetyltransferase activity was over-expressed in the TA muscle of LLC tumor-bearing mice through plasmid transfection. Empty Vector was transfected into the contralateral TA as control. Muscle catabolism measured as UBR2, atrogin1/MAFbx, LC3-II and MHC, was analyzed by Western blotting. (B) Cancer-induced muscle loss is dependent on p300 with intact acetyltransferase activity. TA of mice described in (A) was excised and weighed. * p<0.05 signifies a statistically significant difference after One-way ANOVA analyses or Paired t-test (A and B).