Fig. 1.

Molecular identity of SERT+ neurons in the prefrontal cortex (PFC). a Transient SERT expression in the PFC during postnatal development revealed by in situ hybridization on coronal sections through the frontal pole (postnatal ages (P): 4, 7, 10, and 14). b Immunolabeling against Ctip2 (layer 5), Foxp2 (layer 6), and GFP (SERT^Cre/+) in the PFC. b’ SERT-GFP neurons often colocalize with Ctip2 (arrowheads), Foxp2 (white arrows), or both (yellow arrows). c GFP-expressing neurons from the PFC of SERT^Cre/+:RCE mice were dissected and subsequently isolated using FACS (in c', individual cells indicated by arrows). d Expression levels of monoamine-related transcripts in isolated PFC SERT-GFP neurons from (c') represented by the normalized read counts obtained after deep transcriptome sequencing. e Expression levels of cortical layer-specific molecular markers after transcriptome analysis indicating an enrichment of deep layer markers (layers 5 and 6) in PFC SERT-GFP neurons