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. 2019 Mar 27;10:556. doi: 10.3389/fimmu.2019.00556

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Copyright © 2019 Pradier, Papaserafeim, Li, Rietveld, Kaestel, Gruaz, Vonarburg, Spirig, Puga Yung and Seebach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comprehensive overview of the inhibitory effects of ISD and IVIg on NK cell functions. Direct cytotoxicity was tested following incubation overnight with IL2 (50 U/ml) and IL12 (0.5 ng/ml) in the presence or not of ISD and IVIg against K562 target cells using ⁵¹[Cr]-release and DELFIA cytotoxicity assays. Proliferation was tested following 5d incubation with IL2 (50 U/ml) by ³[H]-incorporation. ADCC was tested using porcine endothelial (PED) target cells coated with human anti-pig antibodies in ⁵¹[Cr]-release and DELFIA cytotoxicity assays. IFNγ production and release was tested following incubation overnight with IL2/IL12 followed by stimulation with K562 cells by intracellular flow cytometry and ELISA. *MPA only showed inhibitory effect on IFNγ production in the CD56^bright NK cell subpopulation. ^#IVIg, enhances IFNγ production and release; opposite to ISD.