Skip to main content
. 2019 Mar 27;10:189. doi: 10.3389/fphys.2019.00189

FIGURE 3.

FIGURE 3

GGF2 treatment enhances Akt phosphorylation at T308 and S473 sites in healthy ventricular myocytes, which is blunted by ErbB receptor blockade (afatinib). Top panels: representative Western blot. Bottom panels: Mean ± SE of protein expression (values expressed relative to basal; n = 3–4/group), normalized to calsequestrin (A,B) or total expression (C,D); #P < 0.05 vs. basal; ∗P < 0.05 vs. GGF2. (E) Total Akt protein expression upon insulin and GGF2 treatment of ventricular myocytes. Top panel: representative Western blot. Bottom panel: Mean ± SE of protein expression (values expressed relative to basal; n = 4–5/group); #P < 0.05 vs. basal. Methods: Western blotting from total lysate of isolated rat ventricular myocytes incubated without (i.e., basal, −) or with (+) insulin or GGF2 (100 ng/ml). For (A–E), the same membrane was probed for the indicated proteins, with calsequestrin used as the loading control.