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. 2019 Mar 27;10:189. doi: 10.3389/fphys.2019.00189

FIGURE 5.

FIGURE 5

Similar to insulin, acute GGF2 treatment stimulates phosphorylation of PDK-1 in healthy ventricular myocytes. Top panels: representative Western blot. Bottom panels: Mean ± SE of phosphorylated protein expression (values expressed relative to basal), normalized to calsequestrin (A) or total protein expression (C); n = 4–9/group; #P < 0.05 vs. basal. Methods: Western blotting from total lysate of isolated rat ventricular myocytes incubated without (i.e., basal) or with insulin or GGF2 (100 ng/ml). (B) Total protein expression of PDK-1 upon insulin and GGF2 treatment of ventricular myocytes. Top panels: representative Western blot. Bottom panels: Mean ± SE of protein expression (values expressed relative to basal); n = 7–17/group; #P < 0.05 vs. basal. Methods: Western blotting from total lysate of isolated rat ventricular myocytes incubated without (i.e., basal) or with insulin or GGF2 (100 ng/ml). For (A–C), the same membrane was probed for the indicated proteins, with calsequestrin used as the loading control.