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. 2019 Mar 27;10:189. doi: 10.3389/fphys.2019.00189

FIGURE 8.

FIGURE 8

GGF2 treatment partially rescues impaired GLUT trafficking via an AS160 dependent pathway during MI. (A) Top panels: representative Western blot. Bottom Panels: Mean ± SE of cell surface GLUT4 protein content in myocytes from MI and age-matched control rats; values normalized to control basal (n = 2–4/group); #P < 0.05 vs. control basal, ∗P < 0.05 vs. MI Basal, †P < 0.05 vs. same treatment in Controls. Methods: Photolabeled biotinylated assay in isolated rat ventricular myocytes incubated without (i.e., basal, −) or with (+) insulin or GGF2 (100 ng/ml). L, labeled (cell surface fraction); UL, unlabeled (intracellular fraction); Con, control. (B) GLUT4 trafficking to the cell surface significantly correlates with AS160 activation in myocytes from healthy and MI rats following incubation with insulin or GGF2. Scatterplot and linear regression of myocardial cell surface GLUT4 content (dependent variable) and AS160 phosphorylation (independent variable) in myocytes of control and MI rats (n = 2–4/group) under basal conditions or after in vitro insulin or GGF2 treatment; P < 0.0001; R2 = 0.5482; Y = 0.6401 X + 0.5165. RU, relative units.