Table 1.
PCR primers used in this study.
| Gene name | Primer | Sequence |
|---|---|---|
| NKG2D RECEPTOR | ||
| FL_NKG2D | Forward | GCTGTATTCCTAAACTCATTATTCAACC |
| Reverse | CTGCCAAGATCCATTTGTTG | |
| Tr_NKG2D | Forward | TTCTGCTGCTTCATCGCTGT |
| Reverse | TGGACTAATAGCAAAAATGTGACAA | |
| Both_NKG2D | Forward | CCTCTCTGCGGTAGACGTG |
| Reverse | GACATCTTTGCTTTTGCCATC | |
| NKG2D LIGANDS | ||
| MICA | Forward | AGGGTCTGTGAGATCCATGAAGAC |
| Reverse | CCTGACGTTCATGGCCAAGG | |
| MICB | Forward | ACCTTGGCTATGAACGTCACA |
| Reverse | CCCTCTGAGACCTCGC | |
| ULBP-2 | Forward | GCAAGGATGTCTTGTGAGCA |
| Reverse | GGCCACAACCTTGTCATTCT | |
| HOUSEKEEPING GENES | ||
| β2-microglobulin | Forward | GGGTTTCATCCATCCATCCGACA |
| Reverse | ACACGGCAGGCATACTCATC | |
| β-actin | Forward | CTGAACCCCAAGGCCAAC |
| Reverse | CAGAGGCGTACAGGGATAGC | |
| 18S RNA | Forward | GACTCAACACGGGAAACCTC |
| Reverse | AGACAAATCGCTCCACCAAC | |
To analyze the mRNA expression of NKG2D receptor and ligand following PCR primers were used. Housekeeping genes were used as internal control. PCR primers were designed using the Web-based primer3 software (http://primer3.wi.mit.edu/) and purchased from TIB MOLBIOL.