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. 2019 Apr 2;10(2):e00128-19. doi: 10.1128/mBio.00128-19

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Copyright © 2019 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Fut8^+/− maternal mouse-fed neonates showed a lower proportion of splenic CD19⁺ CD69⁺ B lymphocytes and attenuated humoral immune response. (A) Flow cytometry analysis of the proportion of spleen cells in Fut8^+/+ and Fut8^+/− maternal mouse-fed offspring. Numbers indicate the percentage of the indicated cell groups in total spleen cells. Ten thousand events were acquired for each analysis. Data are representative of three independent experiments and are shown as mean ± SEM (*, P < 0.05). (B) Experimental design. Fut8^+/+ and Fut8^+/− maternal mouse-fed offspring were immunized by abdominal cavity injection with 50 µg of OVA mixed with an equal volume of complete Freund’s adjuvant (CFA). Two weeks later, mice were immunized with 50 µg of OVA by subcutaneous injection. Mice sera were collected before and 14 days post-OVA immunization. (C) Comparison of the levels of serum IgG in Fut8^+/+ and Fut8^+/− maternal mouse-fed offspring after immunization. The concentrations of IgG in the sera of mice (n = 3/group) were measured by enzyme-linked immunosorbent assay (ELISA) using mouse MAb isotyping reagents. Data are shown as mean ± SEM (n = 3; *, P < 0.05). (D) Flow cytometry analysis of the CD3⁺ and CD19⁺ cell proportions in spleen of Fut8^+/+ and Fut8^+/− maternal mouse-fed offspring after immunization. Data are shown as mean ± SEM (n = 3; *, P < 0.05). (E) Experimental design. Fut8^+/+ and Fut8^+/− maternal mouse-fed offspring were orally administered with a minimally effective dose of antibiotics (mixAbx: ampicillin, neomycin, and metronidazole at 40 mg/liter and vancomycin at 20 mg/liter) from the 10th day to the 21st day after birth. (F) Flow cytometry analysis of the CD3⁺ and CD19⁺ cell proportions in spleen of Fut8^+/+ and Fut8^+/− maternal mouse-fed offspring after immunization. Data are shown as mean ± SEM (n = 3). n.s., no significant differences were detected between groups.