FIG 6.

Core-fucosylated oligosaccharides promote the growth of tested Bifidobacterium and Lactobacillus strains (A) The basic fucosyl structure on milk N-glycan: Fuc-α1,6-GlcNAc-GlcNAc and Fuc-α1,6-GlcNAc. (B) The synthetic process of Fuc-α1,6-GlcNAc-GlcNAc (35). (C) The synthetic process of Fuc-α1,6-GlcNAc (details in Materials and Methods). (D) The growth curves of Bifidobacterium and Lactobacillus strains when cultured in liquid media supplemented with chemically synthesized Fuc-α1,6-GlcNAc-GlcNAc as the sole carbon source. (E) The growth curves of Bifidobacterium and Lactobacillus strains when cultured in liquid media supplemented with chemically synthesized Fuc-α1,6-GlcNAc as the sole carbon source. The oligosaccharide concentration was 10 mM. Growth was monitored by measuring the OD₅₄₆. Three technical replicates were performed for each strain. *, P < 0.05, **, P < 0.01, and ***, P < 0.001, by two-way ANOVA.