FIG 5.

Respiratory heterogeneity provides a fitness advantage during urinary tract infection. (A and B) Pie charts depicting relative abundance of cydA, cyoA, and appC transcripts detected using RT-qPCR in planktonic cultures grown at 21% oxygen in the manner used to prepare cultures to inoculate mice (A), as well as planktonic cultures grown at 4% oxygen (B). Data are representative of three biological replicates. (C to G) PNA-FISH was used to detect quinol oxidase transcripts from cultures used to inoculate mice. Data are representative of three biological replicates. (H to L) PNA-FISH was used to detected quinol oxidase transcripts in the urine of mice infected with UTI89. Urine was pooled from 20 mice. (M) Graph depicting bladder titers obtained from mice infected with UTI89 or quinol oxidase mutant strains at 24 h postinfection. Each point represents a mouse. UTI89 and ΔcydAB strains are representative of two independent experiments. ΔcyoAB and ΔappBC strains are representative of one experiment. Statistical analysis was performed in GraphPad Prism using a two-tailed Mann-Whitney test. Line represents geometric mean. ***, P < 0.001.