Table 1.

Specific primers and probes used in the qPCR.

Periodontal Pathogens	Primer/TaqMan probe sequence (5′-3′)
Aggregatibacter actinomycetemcomitans
Forward primer	5′- GAACCTTACCTACTCTTGACATCCGAA-3′
Reverse primer	5′-TGCAGCACCTGTCTCAAAGC-3′
Probe	FAM-AGAACTCAGAGATGGGTTTGTGCCTTAGGG-TAMRA
Porphyromonas gingivalis
Forward primer	5′-GCGCTCAACGTTCAGCC-3′
Reverse primer	5′-CACGAATTCCGCCTGC-3′
Probe	FAM-CACTGAACTCAAGCCCGGCAGTTTCAA-TAMRA
Prevotella intermedia
Forward primer	5′-CGGTCTGTTAAGCGTGTTGTG-3′
Reverse primer	5′-CACCATGAATTCCGCATACG-3′
Probe	FAM-TGGCGGACTTGAGTGCACGC-TAMRA
Tannerella forsythensis
Forward primer	5′-GGGTGAGTAACGCGTATGTAACCT-3′
Reverse primer	5′-ACCCATCCGCAACCAATAAA-3′
Probe	FAM- CCCGCAACAGAGGGATAACCCGG-TAMRA
SYBR
Forward primer	5′- ACT-CCT-ACG-GGA-GGC-AGC-AG-3′
Reverse primer	5′-ATTACCGCGGCTGCTGG-3′