Table 3.
Free-running locomotor rhythmsa
| Temperature | Genotype | Period ± SEM (h) | Power ± SEM | n | %R |
|---|---|---|---|---|---|
| 25°C | Pdf-GAL4, UAS-dcr2/+ | 24.5 ± 0.08 | 101.8 ± 9.42 | 32 | 62.5 |
| Pdf-GAL4, UAS-dcr2/UAS-Rnb RNAi23099 | 26.6 ± 0.09*** | 108.9 ± 7.04 | 29 | 79.3 | |
| tim-GAL4/+ | 24.4 ± 0.05 | 119.2 ± 5.91 | 26 | 92.3 | |
| tim-GAL4/+; UAS-Rnb RNAi23099 | 26.3 ± 0.04*** | 136.9 ± 4.68 | 32 | 100 | |
| tub-GAL4/+ | 23.7 ± 0.04 | 156.8 ± 8.4 | 62 | 96.7 | |
| tub-GAL4/UAS-Rnb RNAi23099 | 25.2 ± 0.07*** | 98.5 ± 8.5 | 40 | 80.0* | |
| Pdf-Gal4/+ | 24.0 ± 0.06 | 84.2 ± 5.0 | 61 | 95.0 | |
| UAS-RnB::Venus/+ | 23.6 ± 0.04 | 94.3 ± 6.2 | 57 | 96.5 | |
| Pdf-GAL4, UAS-Rnb::Venus | 24.1 ± 0.07 | 78.9 ± 5.7 | 61 | 85.3 | |
| UAS-Rnb RNAi22573/+ | 23.9 ± 0.06 | 122.4 ± 9.8 | 63 | 87.3 | |
| Pdf-GAL4/UAS-Rnb RNAi22573 | 24.5 ± 0.02**** | 174.9 ± 4.9 | 122 | 96.7 | |
| w1118 | 23.7 ± 0.17 | 111.5 ± 7.20 | 63 | 93.6 | |
| Rnbf04616/+ | 24.0 ± 0.21 | 59.9 ± 3.11 | 64 | 70.3*** | |
| Rnbf04616 | 25.2 ± 0.76 | 18.8 ± 2.25 | 89 | 14.6*** | |
| Rnbf04616/Df | 23.5 ± 0.21 | 80.5 ± 36.99 | 16 | 18.8*** | |
| Rnbf05400/+ | 23.8 ± 0.9 | 60.2 ± 2.59 | 64 | 67.2*** | |
| Rnbf05400 | 25.8 ± 0.76* | 22.6 ± 3.17 | 90 | 17.8*** | |
| Rnbf05400/Df | 23.2 ± 0.2 | 64.5 ± 10.53 | 63 | 23.8*** | |
| Rnbf04616/Rnbf05400 | 23.3 ± 0.1 | 111.8 ± 23.9 | 31 | 35.5*** | |
| Df/+ | 23.4 ± 0.1 | 74.9 ± 12.20 | 10 | 50.0*** | |
| 18°C → 29°C | w1118 | 23.7 ± 0.08 | 147.1 ± 9.69 | 32 | 84 |
| Adult-only | Pdf-GAL4/+; tub-GAL80ts/+ | 24.3 ± 0.06 | 175.7 ± 6.40 | 62 | 95.2 |
| gal1118/ tub-GAL80ts | 23.5 ± 0.04 | 134.0 ± 6.89 | 58 | 89.7 | |
| UAS-Rnb RNAi23099/Pdf-GAL4; tub-GAL80ts/+ | 26.0 ± 0.07*** | 109.8 ± 6.90 | 31 | 100 | |
| UAS-Rnb RNAi23099/+; gal1118/tub-GAL80ts/+ | 25.4 ± 0.19*** | 87.3 ± 7.46 | 28 | 85.7 | |
| UAS-miR-unf/+; gal1118/ tub-GAL80ts | 25.4 ± 0.15*** | 127.5 ± 5.70 | 87 | 97.7 | |
| UAS-miR-unf/UAS-Rnb RNAi23099; gal1118/tub-GAL80ts | 26.1 ± 0.11*** | 118.6 ± 5.15 | 61 | 93.4 | |
| 29°C → 18°C | Pdf-GAL4/+; tub-GAL80ts/+ | 24.1 ± 0.06 | 94.1 ± 6.93 | 62 | 80.6 |
| Developmental | UAS-Rnb RNAi23099/Pdf-GAL4; tub-GAL80ts/+ | 24.4 ± 0.08** | 93.3 ± 7.33 | 31 | 93.5 |
an, Number of flies; %R, % of rhythmic flies. Rhythmicity was compared with the driver control or with control genotypes (w1118 for Rnb mutants) by a χ2 test.
Periods were compared with the controls (Student's t test): *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.