Table 1.
Summary of statistical tests
Figure | Test | Statistical value | p-value | n (no. of animals) |
Post hoc comparisons |
|||
---|---|---|---|---|---|---|---|---|
Groups compared | Mean 1 | Mean 2 | Significance | |||||
2C | One-way ANOVA | F(3,22) = 16.68 | <0.0001 | 7 (control) | Control vs low RhebCA | 95.6 | 127.8 | * |
8 (low RhebCA) | Control vs int RhebCA | 95.6 | 143.7 | ** | ||||
6 (int RhebCA) | Control vs high RhebCA | 95.6 | 180.6 | **** | ||||
5 (high RhebCA) | Low vs int RhebCA | 127.8 | 143.7 | ns | ||||
Low vs high RhebCA | 127.8 | 180.6 | ** | |||||
Int vs high RhebCA | 143.7 | 180.6 | * | |||||
2E | One-way ANOVA | F(3,22) = 14.92 | <0.0001 | 7 (control) | Control vs low RhebCA | 88.9 | 156.4 | ** |
8 (low RhebCA) | Control vs int RhebCA | 88.9 | 143.9 | * | ||||
6 (int RhebCA) | Control vs high RhebCA | 88.9 | 207.9 | **** | ||||
5 (high RhebCA) | Low vs int RhebCA | 156.4 | 143.9 | ns | ||||
Low vs high RhebCA | 156.4 | 207.9 | * | |||||
Int vs high RhebCA | 143.9 | 207.9 | * | |||||
2F | Pearson's product–moment | r = 0.8501 | <0.0001 | 7 (control) | ||||
correlation | 8 (low RhebCA) | |||||||
6 (int RhebCA) | ||||||||
5 (high RhebCA) | ||||||||
2H | One-way ANOVA | F(3,22) = 284.6 | <0.0001 | 7 (control) | Control vs low RhebCA | 82.3 | 232.9 | **** |
8 (low RhebCA) | Control vs int RhebCA | 82.3 | 390.9 | **** | ||||
6 (int RhebCA) | Control vs high RhebCA | 82.3 | 486.0 | **** | ||||
5 (high RhebCA) | Low vs int RhebCA | 232.9 | 390.9 | **** | ||||
Low vs high RhebCA | 232.9 | 486.0 | **** | |||||
Int vs high RhebCA | 390.9 | 486.0 | **** | |||||
2I | Pearson's product–moment | r = 0.8257 | <0.0001 | 7 (control) | ||||
correlation | 8 (low RhebCA) | |||||||
6 (int RhebCA) | ||||||||
5 (high RhebCA) | ||||||||
3D | One-way ANOVA | F(3,22) = 17.77 | <0.0001 | 7 (control) | Control vs low RhebCA | 99.6 | 94.9 | ns |
8 (low RhebCA) | Control vs int RhebCA | 99.6 | 56.0 | **** | ||||
6 (int RhebCA) | Control vs high RhebCA | 99.6 | 62.3 | *** | ||||
5 (high RhebCA) | Low vs int RhebCA | 94.9 | 56.0 | *** | ||||
Low vs high RhebCA | 94.9 | 62.3 | ** | |||||
Int vs high RhebCA | 56.0 | 62.3 | ns | |||||
3F | Two-way repeated- | Interaction | <0.0001 | 7 (control) | See graph for relevant significant | |||
measures ANOVA | F(27,198) = 7.371 | 8 (low RhebCA) | post hoc comparisons | |||||
Row effect: F(9,198) = 42.96 | <0.0001 | 6 (int RhebCA) | ||||||
Column effect: F(3,22) = 0.6691 | 0.5800 | 5 (high RhebCA) | ||||||
Subjects (matching): F(22,198) = 6.304e-006 | >0.9999 | |||||||
4B | Spearman's rank–order | r = 0.8709 | <0.0001 | 7 (control) | ||||
correlation | 8 (low RhebCA) | |||||||
7 (int RhebCA) | ||||||||
12 (high RhebCA) | ||||||||
4C | Spearman's rank–order | r = 0.6818 | 0.0018 | 8 (low RhebCA) | ||||
correlation | 6 (int RhebCA) | |||||||
4 (high RhebCA) | ||||||||
4D | Spearman's rank–order | r = 0.8693 | <0.0001 | 8 (low RhebCA) | ||||
correlation | 6 (int RhebCA) | |||||||
4 (high RhebCA) | ||||||||
4E | Mann-Whitney U | U = 15 | 0.0208 | 7 (int RhebCA) | ||||
12 (high RhebCA) | ||||||||
4F | Mann-Whitney U | U = 27 | 0.4240 | 6 (int RhebCA) | ||||
12 (high RhebCA) | ||||||||
5A | One-way ANOVA | F(3,22) = 4.783 | 0.0103 | 7 (control) | Control vs low RhebCA | 74.4 | 64.5 | ns |
8 (low RhebCA) | Control vs int RhebCA | 74.4 | 138.5 | * | ||||
6 (int RhebCA) | Control vs high RhebCA | 74.4 | 88.6 | ns | ||||
5 (high RhebCA) | Low vs int RhebCA | 64.5 | 138.5 | ** | ||||
Low vs high RhebCA | 64.5 | 88.6 | ns | |||||
Int vs high RhebCA | 138.5 | 88.6 | ns | |||||
5B | Spearman's rank–order | r = 0.4294 | 0.0754 | 8 (low RhebCA) | ||||
correlation | 6 (int RhebCA) | |||||||
4 (high RhebCA) | ||||||||
6B | One-way ANOVA | F(2,18) = 6.166 | 0.0091 | 7 (control) | Control vs low RhebCA | 131.7 | 144.7 | ns |
8 (low RhebCA) | Control vs int RhebCA | 131.7 | 167.1 | ** | ||||
6 (int RhebCA) | Low vs int RhebCA | 144.7 | 167.1 | ns | ||||
6C | Spearman's rank–order | r = 0.5502 | 0.0441 | 8 (low RhebCA) | ||||
correlation | 6 (int RhebCA) | |||||||
6D | Two-way repeated- | Interaction: | 0.0414 | 7 (control) | Ipsi-contra | |||
measures ANOVA | F(2,16) = 3.913 | 8 (low RhebCA) | Control | 131.7 | 136.0 | ns | ||
Row effect: | 0.0494 | 4 (int RhebCA) | Low | 144.7 | 138.2 | ns | ||
F(1,16) = 4.522 | Intermediate | 179.3 | 163.5 | * | ||||
Column effect: | 0.0006 | Ipsi | ||||||
F(2,16) = 12.05 | Control vs low | 131.7 | 144.7 | ns | ||||
Subjects (matching): | 0.0022 | Control vs int | 131.7 | 179.3 | **** | |||
F(16,16) = 4.522 | Low vs int | 144.7 | 179.3 | *** | ||||
Contra | ||||||||
Control vs low | 136.0 | 138.2 | ns | |||||
Control vs int | 136.0 | 163.5 | ** | |||||
Low vs int | 138.2 | 163.5 | * | |||||
6E | One-way ANOVA | F(2,18) = 7.472 | 0.0043 | 7 (control) | Control vs low RhebCA | 49.7 | 51.6 | ns |
8 (low RhebCA) | Control vs int RhebCA | 49.7 | 72.0 | ** | ||||
6 (int RhebCA) | Low vs int RhebCA | 51.6 | 72.0 | * |
*p < 0.05,
**p < 0.01,
***p < 0.001,
****p < 0.0001.