Figure 1.

Transplanted MGE-derived CINs successfully differentiate and disperse in the host neocortex. A, Experimental strategy showing that transgenic mice carrying Vgat floxed allele were crossed with Nkx2.1-cre mouse driver line to conditionally knock out Vgat from MGE-derived interneurons. B, Cells were dissected from the MGE at E13.5, dissociated, and transplanted into the visual cortex of P2–P6 hosts. MGE transplantation recipient brains were examined for ODP at 33–35 DAT. C, Coronal sections were stained for Td-tomato, SST, and PV, which shows dispersion in the host visual cortex. D, Representative magnified image of the visual cortex showing colocalization between td-Tomato and SST in Vgat^−/− MGE cells (left) and td-Tomato and PV in Vgat^−/− MGE cells (right). E, Percentage of transplanted SST (top) and PV (bottom) interneurons that express tdTomato in anatomically isolated MGE transplants.