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Western blotting. Cell lysates of CHO-K1 and CHO/tasPDPN (10 μg) were electrophoresed and transferred onto PVDF membranes. The membranes were incubated with l μg/mL of PMab-233, anti-PA16 tag (NZ-1), or anti-β-actin and subsequently, with peroxidase-conjugated anti-mouse or -rat IgG.
