Figure 5.

Effects of Api pretreatment on cardiomyocyte viability and LDH activity after SI/R injury. The primary cardiomyocytes were cultured with different concentrations of pretreated Api (10, 20, 40, and 80 μM) for 24 h, then normally cultured for 19 h prior to SI/R injury. Api pretreatment increased cell viability (a) and reduced LDH activity (b) in a concentration-dependent manner. Values are presented as mean ± S.E.M. of 8 individual experiments. (a) p < 0.01 vs. control group; (b) p < 0.01 vs. SI/R group.