Figure 1.

RANKL promoter probes containing SNPs bind nuclear proteins with different affinities. A nuclear extract of hFOB cells was incubated with biotin-labeled probes in the presence or absence of a 100-fold excess of unlabeled probes, 2 μg anti-Sp1 antibody (S), or mouse IgG (m). After 30 min, the reaction mixture was analyzed by EMSA. (a) Nuclear proteins formed 3 complexes with the -693G probe, but none with the -693C probe. (b) Nuclear proteins and DNA probes formed more complexes with the −290T probe than with the -290C probe at the indicated region. Asterisks indicate protein-DNA complexes.