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. 2019 Mar 27;10:575. doi: 10.3389/fimmu.2019.00575

Figure 6.

Figure 6

The balance between retention and exit signals is altered in apoE−/− mice LNs. (A) Immunoreactivity for LYVE-1, B220, and CD45.1 was examined on LN sections from long-term adoptive transfer at t = 0 and t = 20 h to determine the localization of CD45.1 transferred lymphocytes in enlarged LNs from apoE−/− mice. Images are representative of three to four independent experiments (n = 3–4 mice per group) (B) CCL21 protein content was analyzed in homogenates of 22 to 28 weeks-old WT and apoE−/− mice LNs by ELISA. (C) Immunoreactivity for CCL21 and LYVE-1 was examined in LN sections from 22 to 28 weeks-old WT and apoE−/− mice. Images are representative of three to four independent experiments (n = 3–5 mice per group) (D) S1P content was analyzed in efferent lymph of 22 to 28 weeks-old WT and apoE−/− mice by mass spectrometry. (E) Quantitative RT-PCR analysis was performed on whole LN RNA to examine expression of Sph1k and Spgl1 in WT and apoE−/− mice at 22 to 28 weeks of age. n = 6–10 mice per group; *p < 0.05.