Table 2.
Linear range and correlation coefficient and sensitivity of 10 amino acids in ESI+ mode.
| Amino acids | Linear range (ng/mL) | r | LOD | LOQ | ||||
|---|---|---|---|---|---|---|---|---|
| Content | S/N average | RSD% (n = 5) | Content | S/N average | RSD% (n = 5) | |||
| Tryptophan | 6.04-386.25 | 0.9985 | 100 pg/mL | 5.27 | 9.67 | 1000 pg/mL | 14.58 | 10.89 |
| Tyrosine | 2.50-160.00 | 0.9844 | 15 pg/mL | 4.10 | 14.37 | 150 pg/mL | 12.61 | 11.71 |
| Phenylalanine | 0.98-62.40 | 0.9995 | 2 pg/mL | 12.02 | 12.53 | 20 pg/mL | 13.18 | 16.91 |
| Glutamate | 37.19-2380.00 | 0.9995 | 50 pg/mL | 6.44 | 13.31 | 500 pg/mL | 14.83 | 12.77 |
| Glutamine | 177.19-11340.00 | 0.9834 | 5 pg/mL | 4.64 | 19.54 | 50 pg/mL | 17.72 | 10.14 |
| Isoleucine | 1.23-78.60 | 0.9960 | 3.5 pg/mL | 5.04 | 15.19 | 35 pg/mL | 12.97 | 5.13 |
| Leucine | 0.90-57.50 | 0.9995 | 0.7 pg/mL | 6.57 | 16.79 | 7 pg/mL | 14.30 | 12.86 |
| Taurine | 43.24-2767.50 | 0.9980 | 110 pg/mL | 3.16 | 13.19 | 1100 pg/mL | 17.05 | 14.49 |
| Valine | 1.58-101.00 | 0.9995 | 3.5 pg/mL | 4.65 | 12.14 | 35 pg/mL | 10.95 | 9.52 |
| Alanine | 18.28-1170.00 | 0.9995 | 100 pg/mL | 5.35 | 14.55 | 1000 pg/mL | 12.69 | 15.76 |
S/N was calculated as RMS (root mean square) S/N.