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. 2019 Mar 15;20(4):e46293. doi: 10.15252/embr.201846293

Figure EV2. cGAS contributes to sensing of eCDNs.

Figure EV2

  1. Western blot detection of indicated proteins in lysates of HEK293T cells stably transfected with pcDNA3.1‐HA (HA), HA‐STING, and HA‐STING + HA‐cGAS. Data are representative of three independent experiments.
  2. Frequencies of FITC+ WT and CGAS KO THP‐1 cells stimulated with FITC‐ecGAMP (5 μg/ml) for indicated times.
  3. qRT–PCR detection of Ifnb1 mRNA in WT, Cgas KO, or Cgas KO complemented with cGAS (Cgas KO+cGAS) RAW264.7 cells stimulated with indicated eCDNs (5 μg/ml) or transfected with ISD.
  4. qRT–PCR detection of IFNB1 mRNA in HEK293T cells stably transfected with both HA‐STING and HA‐cGAS stimulated with either ecGAMP or icGAMP at the indicated concentrations (μg/ml) for 24 h.
Data information: Data are means + SD averaged from at least three independent experiments performed with technical triplicates. Each symbol in panels (C, D) represents the mean of technical triplicates. Two‐way ANOVA followed by Bonferroni's post hoc test was used for statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001.Source data are available online for this figure.