Figure EV4. cGAS facilitates eCDN‐induced type I IFN response dispensable of resynthesis of cGAMP by cGAS .

• A
  Western blot detection of indicated proteins in lysates of Sting KO RAW264.7 cells (Sting KO) and Sting KO RAW264.7 cells complemented with WT mouse STING (Sting KO+mSTING) or mSTING^R231A (Sting KO+ mSTING^R231A). Data are representative of three independent experiments.
• B, C
  qRT–PCR detection of Ifnb1 mRNA (B) or ELISA detection of IFNβ in the supernatants (C) of WT, Sting KO, and Sting KO+ mSTING^R231A RAW264.7 cells stimulated with ecGAMP (5 μg/ml) or ec‐di‐GMP (5 μg/ml) or transfected with ISD.
• D
  Western blot detection of indicated proteins in lysates of CGAS KO THP‐1 cells (CGAS KO) and CGAS KO THP‐1 cells complemented with WT cGAS (CGAS KO+cGAS) or cGAS^{E225A D227A} (CGAS KO+cGAS^{E225A D227A}). Data are representative of three independent experiments.
• E, F
  qRT–PCR detection of IFNB1 mRNA (D) or ELISA detection of IFNβ in the supernatants (E) of WT, CGAS KO, and cGAS KO+cGAS^{E225A D227A} THP‐1 cells stimulated with ecGAMP at indicated concentrations.

Data information: Data (B, C, E, F) are means + SD averaged from at least two independent experiments performed in technical duplicates or triplicate and each symbol represents mean of technical replicates. Two‐way ANOVA followed by Bonferroni's post hoc test was used for statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.Source data are available online for this figure.