Figure 5. cGAS facilitates eCDN detection in macrophages.

• A
  qRT–PCR detection of IFNB1 mRNA in WT and CGAS KO THP‐1 cells stimulated with indicated eCDNs (5 μg/ml) or transfected with ISD or poly (I:C) for 4 h.
• B
  ELISA detection of IFNβ in supernatants of WT and CGAS KO THP‐1 cells stimulated with indicated eCDNs (5 μg/ml) or transfected with ISD or poly(I:C) for 4 h.
• C
  qRT–PCR detection of Ifnb1 mRNA in mBMDMs from WT and Cgas ^−/− (Cgas KO) mice stimulated with indicated eCDNs (5 μg/ml) or transfected with ISD or poly(I:C) for 4 h.
• D
  ELISA detection of IFNβ in supernatants of mBMDMs from WT and Cgas KO mice stimulated with indicated eCDNs (5 μg/ml) or transfected with ISD or poly(I:C) for 4 h.
• E
  qRT–PCR detection of IFNB1 mRNA in WT and CGAS KO THP‐1 cells stimulated with ecGAMP (5 μg/ml) or icGMAP (0.1 μg/ml) or transfected with ISD.
• F
  qRT–PCR detection of Ifnb1 mRNA in mBMDMs from WT and Cgas KO stimulated with ecGAMP (5 μg/ml) or icGMAP (0.1 μg/ml) or transfected with ISD.
• G
  Western blot detection of indicated proteins in lysates of mBMDMs from WT and Cgas KO mice stimulated with ecGAMP (5 μg/ml) or icGAMP (0.1 μg/ml) or transfected with ISD. Data are representative of three independent experiments.
• H, I
  qRT–PCR detection of IFNB1 mRNA (H) and ELISA detection of IFNβ in supernatants (I) of WT, CGAS KO, CGAS KO complemented with cGAS (CGAS KO+cGAS) or CGAS KO stably transfected with the empty vector (CGAS KO+Vector) THP‐1 cells stimulated with ecGAMP (5 μg/ml).

Data information: Data are means + SD (A–F, H, and I) averaged from at least three independent experiments performed with technical triplicates. Each symbol represents the mean of technical triplicates. Two‐way ANOVA followed by Bonferroni's post hoc test was used for statistical analysis. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.Source data are available online for this figure.