Skip to main content
. 2019 Mar 4;20(4):e46735. doi: 10.15252/embr.201846735

Figure EV1. Mammalian Ccdc78 is not a deuterosome protein.

Figure EV1

  • A, B
    Neither endogenous nor exogenous murine Ccdc78 localized to deuterosomes in mTECs. Cultured mTECs were fixed at day 3 post‐ALI (A) or transfected with lentivirus at day −1 to express GFP‐Ccdc78, followed by fixation at day 3 (B). After immunostaining to visualize the indicated proteins, the samples were imaged with SIM. Parental centrioles (p1; arrows), typical deuterosomes (dt; arrowheads), and typical regions with basal bodies (framed) are magnified 2× to show details. Scale bar, 1 μm.
  • C
    GFP‐Ccdc78 did not localize to Flag‐Deup1‐induced deuterosome‐like structures in U2OS cells. U2OS cells were co‐transfected to express Flag‐Deup1 and GFP‐Ccdc78 for 48 h and fixed for immunostaining. Parental centrioles (p1/p2; arrows) and typical deuterosome‐like structures (dt; arrowheads) are magnified 2× to show details. Scale bar, 1 μm.
  • D
    Ccdc78 and Deup1 had different expression patterns in differentiating mTECs. Cultured mTECs were induced to undergo multiciliation from day 0 and collected at the indicated time. Gapdh served as loading control.