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. 2019 Mar 4;20(4):e46762. doi: 10.15252/embr.201846762

Figure 2. Ash1 SET domain is required to counteract Polycomb repression.

Figure 2

  1. Adult phenotypes of the ash1 22/ash1 9011 flies supplemented with transgenic constructs expressing either the full‐length Ash1 (Ash1FL) or the truncated variants lacking the PHD (Ash1ΔPHD) or the SET (Ash1ΔSET) domains. Note extra hypopleural bristles (red arrows), the third leg (L3) to second leg (L2), haltere (H) to wing, t5–t4 and t6–t5 transformations in the Ash1ΔPHD and the Ash1ΔSET but not in the Ash1FL flies. The latter are evident from the partial loss of pigmentation in t6 and t5, or the appearance of small bristles (trichomas) on t6 of the Ash1ΔPHD and the Ash1∆SET flies in the area that is normally naked (Ash1FL, yellow dashed line). The transformed L3 acquire apical and pre‐apical bristles on the tibia (black triangles) while halteres change shape and acquire rows of bristles (black arrows).
  2. Twofold dilutions of total nuclear protein extracts from the third instar larvae of the ash1 22/ash1 9011 mutants supplemented with various transgenic constructs and wild‐type flies were analysed by Western blot with anti‐Ash1 antibodies. Arrow indicates the position of Ash1 protein. Note that transgenic proteins are expressed at comparable level.
  3. Coomassie staining of SDS–PAGE separated protein extracts from (B) was used to control the loading.

Source data are available online for this figure.