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. 2019 Mar 18;20(4):e47407. doi: 10.15252/embr.201847407

Figure 5. Sphinganine exposure activates molecular signatures of genome instability and senescence in human cardiomyocytes while concomitant HAT inhibition amends the genotoxicity.

Figure 5

  1. hCMs were treated for 3 days with DMSO, DHS and DHS + curcumin (co‐incubation), represented in the scheme. After incubation, cells were harvested for RNA isolation and differentially expressed genes were identified by microarray analysis. Bar graph depicting Gene Ontology (GO) enrichment status for the differentially expressed transcripts upon DHS treatment. Hypergeometric test was used for determining the P‐value. Experiment was performed in biological triplicate for DHS and DHS + Curcumin condition, whereas two biological replicates were used for DMSO condition.
  2. Bar graph representing GO enrichment levels for clinical phenotypes. Hypergeometric test was used for determining the P‐value.
  3. Co‐treatment of curcumin with DHS leads to the activation of DNA repair pathways as shown here by functional enrichment analysis on the differentially expressed transcripts using Metascape. Hypergeometric test was used for determining the P‐value.
  4. Heatmap depicting the expression profile of the markers of DNA damage response in the indicated conditions.
  5. Co‐treatment of curcumin activates DNA repair pathways as depicted here by heatmaps. Color scale represents scaled expression values.