Figure EV2. The localization of Msp1 N‐domain mutants and their effects on GFP‐Pex15Δ30 degradation (associated with Fig 1).

1. The normal localization of Msp1 N‐domain mutants. Z projections and DIC images are shown. Mitochondrial Msp1‐GFP colocalizes with mtBFP. Peroxisomal Msp1 appears as extra‐mitochondrial dots. Scale bar represents 1 μm.
2. Degradation of GFP‐Pex15Δ30 in WT and Msp1 N‐domain mutant cells. Mutants displaying defective degradation are highlighted in red.
3. Degradation of GFP‐Pex15Δ30 in untagged Msp1 N‐domain mutant cells. msp1 ^E193Q ‐FLAG strain is used as a control for Msp1 loss of function.

Data information: In this figure, WT and mutant forms of Msp1‐GFP and Msp1‐FLAG were expressed from the endogenous chromosomal locus, and GFP‐Pex15Δ30 was expressed from a centromeric plasmid under the control of TEF1 promoter.