Figure 5. Pex15 is mislocalized to mitochondria and removed by Msp1 in pex19∆ cells.

1. Chromosomally expressed GFP‐Pex15 driven by endogenous promoter did not mislocalize to mitochondria in GET3 and MSP1 mutant strains.
2. GFP‐Pex15 expressed as in (A) localizes to peroxisome in GET and MSP1 mutant strains. Pex3 was chromosomally tagged with mRFP to visualize peroxisomes.
3. Localization of GFP‐Pex15 expressed as in (A) in the indicated stains. Arrows point to mislocalized GFP‐Pex15 on mitochondria.
4. The percentage of cells with mitochondrial GFP‐Pex15 in the indicated strains. Data values represent means ± SEM from three independent experiments, with at least 100 cells counted in each experiment.
5. The indicated strains were grown in glucose media to log phase and then spotted on SCD or SCEG plates in a 10‐fold serial dilution, and then incubated at indicated temperatures for 2–5 days.

Data information: Scale bars in (A–C) represent 1 μm.