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. 2019 Mar 4;20(4):e46620. doi: 10.15252/embr.201846620

Figure EV3. miR‐132 and its targets p300 and BTAF1 control RP expression.

Figure EV3

  1. mRNA levels of indicated RP transcripts determined by qRT–PCR in MEFs transfected with NTC mimics (white) or miR‐212‐3p mimics (grey). Cultures performed in triplicate. Statistical significance determined by t‐test.
  2. Protein levels determined by Western blot of RPL27 and RPS10 in MEFs transfected with NTC, miR‐132‐3p or miR‐212‐3p mimics for 48 h.
  3. Top enriched molecular function GO terms for miR‐132/212‐3p predicted target genes. Predictions retrieved from Targetscan, total context score < −0.1.
  4. Protein levels determined by Western blot of BTAF1 and p300 in EL4 cells transfected with NTC, miR‐132‐3p, or miR‐212‐3p mimics for 48 h.
Data information: *P < 0.05, **P < 0.01.Source data are available online for this figure.