Figure 1. Defective distribution of endosomal proteins in BAG6‐suppressed cells.

• A
  At 72 h after transfection with siRNA duplexes for BAG6 or control siRNA (10 nM each), the intracellular localization of TfnR in HeLa cells was examined (shown as green). Nuclear DNA was stained with Hoechst 33342 (shown as blue). Control knockdown (left panel), Rab8a knockdown (center panel), and BAG6 knockdown (right panel). Efficacy of endogenous BAG6 knockdown in HeLa cells was verified by Western blot experiments (see Fig EV1D). Scale bar: 10 μm.
• B
  Intracellular localization of Ptc1 (green) in HeLa cells. Nuclei were stained with Hoechst 33342 (shown as blue). See also Fig EV1A and B. Scale bar: 10 μm.
• C, D
  Knockdown of Rab8a (with Rab8a siRNA#1, #2, and #3) or BAG6 (with BAG6 siRNA#1) stimulated the accumulation and stabilization of Ptc1 protein in HEK293 cells. See also Appendix Fig S1B.

Source data are available online for this figure.